The suitability of different cellular in vitro immunotoxicity and genotoxicity methods for the analysis of nanoparticle-induced events

Tobias Pfaller; Renato Colognato; Inge Nelissen; Flavia Favilli; Eudald Casals; Daniëlla Ooms; Hilde Leppens; Jessica Ponti; René Stritzinger; Victor Puntes; Diana Boraschi; Albert Duschl; Gertie J Oostingh

doi:10.3109/17435390903374001

The suitability of different cellular in vitro immunotoxicity and genotoxicity methods for the analysis of nanoparticle-induced events

Tobias Pfaller
, Renato Colognato
, Inge Nelissen
, Flavia Favilli
, Eudald Casals
, Daniëlla Ooms
, Hilde Leppens
, Jessica Ponti
, René Stritzinger
, Victor Puntes
, Diana Boraschi
, Albert Duschl
, Gertie J Oostingh

University of Salzburg

Research output: Contribution to journal › Article › peer-review

Abstract

Suitable assays and test strategies are needed to analyze potential genotoxic and immunotoxic health effects caused by nanoparticle exposure. The development and validation of such methods is challenging because nanoparticles may show unexpected behavior, like aggregation or interference with optical measurements, when routine in vitro assays are performed. In our interdisciplinary study, the effects of inorganic gold (4.5 nm) and iron oxide (7.3 nm) nanoparticles with a narrow size distribution were tested on human cells using different assay systems. The results show that cytotoxicity as well as immunotoxicity and genotoxicity induced by these two inorganic nanoparticles was low or absent when using a panel of cell-based tests in different laboratories. However, several technical issues had to be tackled that were specific for working with nanoparticles. The methods used, their suitability for nanotoxicity testing, and the technical problems encountered are carefully described and discussed in this paper.

Original language	English
Pages (from-to)	52-72
Number of pages	21
Journal	Nanotoxicology
Volume	4
Issue number	1
DOIs	https://doi.org/10.3109/17435390903374001
Publication status	Published - Mar 2010
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cell Line
DNA Damage
Dendritic Cells/cytology
Ferric Compounds/chemistry
Genes, Reporter
Gold/chemistry
Humans
Immunity, Innate/immunology
Immunotoxins/chemistry
Interdisciplinary Studies
Intestinal Mucosa/cytology
Lipopolysaccharides/metabolism
Metal Nanoparticles/chemistry
Monocytes/immunology
Mutagenicity Tests/methods
Oxidative Stress
Reactive Oxygen Species/metabolism

Classification according to Österreichische Systematik der Wissenschaftszweige (ÖFOS 2012)

303012 Health sciences

Applied Research Level (ARL)

Not applicable

Research focus/foci

Applied Health Innovation

Access to Document

10.3109/17435390903374001

Cite this

Pfaller, T., Colognato, R., Nelissen, I., Favilli, F., Casals, E., Ooms, D., Leppens, H., Ponti, J., Stritzinger, R., Puntes, V., Boraschi, D., Duschl, A., & Oostingh, G. J. (2010). The suitability of different cellular in vitro immunotoxicity and genotoxicity methods for the analysis of nanoparticle-induced events. Nanotoxicology, 4(1), 52-72. https://doi.org/10.3109/17435390903374001

@article{090902a0724641fc920c8792da3ba783,

title = "The suitability of different cellular in vitro immunotoxicity and genotoxicity methods for the analysis of nanoparticle-induced events",

abstract = "Suitable assays and test strategies are needed to analyze potential genotoxic and immunotoxic health effects caused by nanoparticle exposure. The development and validation of such methods is challenging because nanoparticles may show unexpected behavior, like aggregation or interference with optical measurements, when routine in vitro assays are performed. In our interdisciplinary study, the effects of inorganic gold (4.5 nm) and iron oxide (7.3 nm) nanoparticles with a narrow size distribution were tested on human cells using different assay systems. The results show that cytotoxicity as well as immunotoxicity and genotoxicity induced by these two inorganic nanoparticles was low or absent when using a panel of cell-based tests in different laboratories. However, several technical issues had to be tackled that were specific for working with nanoparticles. The methods used, their suitability for nanotoxicity testing, and the technical problems encountered are carefully described and discussed in this paper.",

keywords = "Cell Line, DNA Damage, Dendritic Cells/cytology, Ferric Compounds/chemistry, Genes, Reporter, Gold/chemistry, Humans, Immunity, Innate/immunology, Immunotoxins/chemistry, Interdisciplinary Studies, Intestinal Mucosa/cytology, Lipopolysaccharides/metabolism, Metal Nanoparticles/chemistry, Monocytes/immunology, Mutagenicity Tests/methods, Oxidative Stress, Reactive Oxygen Species/metabolism",

author = "Tobias Pfaller and Renato Colognato and Inge Nelissen and Flavia Favilli and Eudald Casals and Dani{\"e}lla Ooms and Hilde Leppens and Jessica Ponti and Ren{\'e} Stritzinger and Victor Puntes and Diana Boraschi and Albert Duschl and Oostingh, \{Gertie J\}",

year = "2010",

month = mar,

doi = "10.3109/17435390903374001",

language = "English",

volume = "4",

pages = "52--72",

journal = "Nanotoxicology",

issn = "1743-5390",

publisher = "Taylor and Francis Ltd.",

number = "1",

}

Pfaller, T, Colognato, R, Nelissen, I, Favilli, F, Casals, E, Ooms, D, Leppens, H, Ponti, J, Stritzinger, R, Puntes, V, Boraschi, D, Duschl, A & Oostingh, GJ 2010, 'The suitability of different cellular in vitro immunotoxicity and genotoxicity methods for the analysis of nanoparticle-induced events', Nanotoxicology, vol. 4, no. 1, pp. 52-72. https://doi.org/10.3109/17435390903374001

TY - JOUR

T1 - The suitability of different cellular in vitro immunotoxicity and genotoxicity methods for the analysis of nanoparticle-induced events

AU - Pfaller, Tobias

AU - Colognato, Renato

AU - Nelissen, Inge

AU - Favilli, Flavia

AU - Casals, Eudald

AU - Ooms, Daniëlla

AU - Leppens, Hilde

AU - Ponti, Jessica

AU - Stritzinger, René

AU - Puntes, Victor

AU - Boraschi, Diana

AU - Duschl, Albert

AU - Oostingh, Gertie J

PY - 2010/3

Y1 - 2010/3

N2 - Suitable assays and test strategies are needed to analyze potential genotoxic and immunotoxic health effects caused by nanoparticle exposure. The development and validation of such methods is challenging because nanoparticles may show unexpected behavior, like aggregation or interference with optical measurements, when routine in vitro assays are performed. In our interdisciplinary study, the effects of inorganic gold (4.5 nm) and iron oxide (7.3 nm) nanoparticles with a narrow size distribution were tested on human cells using different assay systems. The results show that cytotoxicity as well as immunotoxicity and genotoxicity induced by these two inorganic nanoparticles was low or absent when using a panel of cell-based tests in different laboratories. However, several technical issues had to be tackled that were specific for working with nanoparticles. The methods used, their suitability for nanotoxicity testing, and the technical problems encountered are carefully described and discussed in this paper.

AB - Suitable assays and test strategies are needed to analyze potential genotoxic and immunotoxic health effects caused by nanoparticle exposure. The development and validation of such methods is challenging because nanoparticles may show unexpected behavior, like aggregation or interference with optical measurements, when routine in vitro assays are performed. In our interdisciplinary study, the effects of inorganic gold (4.5 nm) and iron oxide (7.3 nm) nanoparticles with a narrow size distribution were tested on human cells using different assay systems. The results show that cytotoxicity as well as immunotoxicity and genotoxicity induced by these two inorganic nanoparticles was low or absent when using a panel of cell-based tests in different laboratories. However, several technical issues had to be tackled that were specific for working with nanoparticles. The methods used, their suitability for nanotoxicity testing, and the technical problems encountered are carefully described and discussed in this paper.

KW - Cell Line

KW - DNA Damage

KW - Dendritic Cells/cytology

KW - Ferric Compounds/chemistry

KW - Genes, Reporter

KW - Gold/chemistry

KW - Humans

KW - Immunity, Innate/immunology

KW - Immunotoxins/chemistry

KW - Interdisciplinary Studies

KW - Intestinal Mucosa/cytology

KW - Lipopolysaccharides/metabolism

KW - Metal Nanoparticles/chemistry

KW - Monocytes/immunology

KW - Mutagenicity Tests/methods

KW - Oxidative Stress

KW - Reactive Oxygen Species/metabolism

U2 - 10.3109/17435390903374001

DO - 10.3109/17435390903374001

M3 - Article

C2 - 20795902

SN - 1743-5390

VL - 4

SP - 52

EP - 72

JO - Nanotoxicology

JF - Nanotoxicology

IS - 1

ER -

The suitability of different cellular in vitro immunotoxicity and genotoxicity methods for the analysis of nanoparticle-induced events

Abstract

UN SDGs

Keywords

Classification according to Österreichische Systematik der Wissenschaftszweige (ÖFOS 2012)

Applied Research Level (ARL)

Research focus/foci

Access to Document

Fingerprint

Cite this