Evaluation of reference intervals of haematological and biochemical markers in an Austrian adolescent study cohort

B. Bogner; K. Schwenoha; M. Vogl; D. Weghuber; C. Roth; U. Kipman; J. Cadamuro; G.J. Oostingh

doi:10.1515/cclm-2018-0715

Evaluation of reference intervals of haematological and biochemical markers in an Austrian adolescent study cohort

B. Bogner
, K. Schwenoha
, M. Vogl
, D. Weghuber
, C. Roth
, U. Kipman
, J. Cadamuro
, G.J. Oostingh

Department of Pediatrics, Paracelsus Medical University
Pädagogische Hochschule Salzburg Stefan Zweig
Department of Laboratory Medicine, Paracelsus Medical University

Research output: Contribution to journal › Article › peer-review

Abstract

Background
Reference intervals are a prerequisite for the interpretation of laboratory data related to diagnostic issues and treatment strategies. In adolescents, biomarker concentrations change with age, necessitating a continuous age-related definition of the reference intervals. The purpose of this pilot study was to evaluate the reference intervals for a healthy population of adolescents in Salzburg and compare these, when possible, with age- and gender-matched published data.

Methods
Anthropometrical parameters and blood samples were collected from adolescents (male and female; 14–17 years) in a school setting. Haematological samples were measured using Sysmex XS-1000i, lipid and carbohydrate metabolism markers as well as enzymes and hormones were determined by Cobas c311, Vitros ECiQ® or ELISA. The reference intervals were calculated according to the CLSI guidelines C28-A3c.

Results
Samples of 102 participants were included. Compared to age- and gender-matched reference intervals, the BMI levels were in the lower normal rage. Most haematological parameters and biomedical makers reveal similar ranges to values published in other studies.

Conclusions
This data analysis allowed for a partial comparison of reference values with published data and enabled a new determination of paediatric reference intervals for an Austrian cohort. © 2019 Barbara Bogner et al., published by De Gruyter, Berlin/Boston.

Original language	English
Pages (from-to)	891-900
Number of pages	10
Journal	Clinical Chemistry and Laboratory Medicine
Volume	57
Issue number	6
DOIs	https://doi.org/10.1515/cclm-2018-0715
Publication status	Published - 30 Oct 2018

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

adolescents
laboratory parameters
paediatrics
post-analytics
reference intervals
adiponectin
alanine aminotransferase
albumin
apolipoprotein A1
apolipoprotein B
aspartate aminotransferase
biochemical marker
C reactive protein
cholesterol
creatinine
cyanocobalamin
cystatin C
ferritin
folic acid
gamma glutamyltransferase
glucose
hemoglobin
hemoglobin A1c
high density lipoprotein cholesterol
insulin
liothyronine
lipoprotein A
low density lipoprotein cholesterol
proinsulin
thyrotropin
triacylglycerol
urea
uric acid
vitamin D
zinc
biological marker
adolescent
alanine aminotransferase blood level
albumin blood level
Article
aspartate aminotransferase blood level
Austrian
basophil
body mass
cholesterol blood level
cohort analysis
controlled study
creatinine blood level
eosinophil count
erythrocyte count
female
ferritin blood level
free liothyronine index
free thyroxine index
gamma glutamyl transferase blood level
glucose blood level
hematocrit
hematological parameters
hemoglobin blood level
human
insulin blood level
leukocyte count
lipoprotein blood level
lymphocyte count
major clinical study
male
mean corpuscular hemoglobin
mean corpuscular volume
monocyte count
neutrophil count
pilot study
platelet count
priority journal
protein blood level
reference value
thyrotropin blood level
triacylglycerol blood level
urea blood level
uric acid blood level
vitamin blood level
zinc blood level
Austria
blood
enzyme assay
enzyme linked immunosorbent assay
normal human
waist hip ratio
Adolescent
Biomarkers
Body Mass Index
Cohort Studies
Enzyme Assays
Enzyme-Linked Immunosorbent Assay
Female
Healthy Volunteers
Humans
Male
Reference Values
Waist-Hip Ratio

Classification according to Österreichische Systematik der Wissenschaftszweige (ÖFOS 2012)

303012 Health sciences

Applied Research Level (ARL)

Not applicable

Research focus/foci

Applied Health Innovation

Access to Document

10.1515/cclm-2018-0715

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85056201265&doi=10.1515%2fcclm-2018-0715&partnerID=40&md5=952ddbceed7a60cac2d0d801651d53ed

Cite this

@article{d4f28aeb8a31475dab284fd700891a11,

title = "Evaluation of reference intervals of haematological and biochemical markers in an Austrian adolescent study cohort",

abstract = "Background Reference intervals are a prerequisite for the interpretation of laboratory data related to diagnostic issues and treatment strategies. In adolescents, biomarker concentrations change with age, necessitating a continuous age-related definition of the reference intervals. The purpose of this pilot study was to evaluate the reference intervals for a healthy population of adolescents in Salzburg and compare these, when possible, with age- and gender-matched published data. Methods Anthropometrical parameters and blood samples were collected from adolescents (male and female; 14{\textendash}17 years) in a school setting. Haematological samples were measured using Sysmex XS-1000i, lipid and carbohydrate metabolism markers as well as enzymes and hormones were determined by Cobas c311, Vitros ECiQ{\textregistered} or ELISA. The reference intervals were calculated according to the CLSI guidelines C28-A3c. Results Samples of 102 participants were included. Compared to age- and gender-matched reference intervals, the BMI levels were in the lower normal rage. Most haematological parameters and biomedical makers reveal similar ranges to values published in other studies. Conclusions This data analysis allowed for a partial comparison of reference values with published data and enabled a new determination of paediatric reference intervals for an Austrian cohort. {\textcopyright} 2019 Barbara Bogner et al., published by De Gruyter, Berlin/Boston.",

keywords = "adolescents, laboratory parameters, paediatrics, post-analytics, reference intervals, adiponectin, alanine aminotransferase, albumin, apolipoprotein A1, apolipoprotein B, aspartate aminotransferase, biochemical marker, C reactive protein, cholesterol, creatinine, cyanocobalamin, cystatin C, ferritin, folic acid, gamma glutamyltransferase, glucose, hemoglobin, hemoglobin A1c, high density lipoprotein cholesterol, insulin, liothyronine, lipoprotein A, low density lipoprotein cholesterol, proinsulin, thyrotropin, triacylglycerol, urea, uric acid, vitamin D, zinc, biological marker, adolescent, alanine aminotransferase blood level, albumin blood level, Article, aspartate aminotransferase blood level, Austrian, basophil, body mass, cholesterol blood level, cohort analysis, controlled study, creatinine blood level, eosinophil count, erythrocyte count, female, ferritin blood level, free liothyronine index, free thyroxine index, gamma glutamyl transferase blood level, glucose blood level, hematocrit, hematological parameters, hemoglobin blood level, human, insulin blood level, leukocyte count, lipoprotein blood level, lymphocyte count, major clinical study, male, mean corpuscular hemoglobin, mean corpuscular volume, monocyte count, neutrophil count, pilot study, platelet count, priority journal, protein blood level, reference value, thyrotropin blood level, triacylglycerol blood level, urea blood level, uric acid blood level, vitamin blood level, zinc blood level, Austria, blood, enzyme assay, enzyme linked immunosorbent assay, normal human, waist hip ratio, Adolescent, Biomarkers, Body Mass Index, Cohort Studies, Enzyme Assays, Enzyme-Linked Immunosorbent Assay, Female, Healthy Volunteers, Humans, Male, Reference Values, Waist-Hip Ratio",

author = "B. Bogner and K. Schwenoha and M. Vogl and D. Weghuber and C. Roth and U. Kipman and J. Cadamuro and G.J. Oostingh",

note = "Cited By :4 Export Date: 14 December 2023 CODEN: CCLMF Correspondence Address: Bogner, B.; Salzburg University of Applied Sciences, Urstein S{\"u}d 1, Austria; email: [email protected] Chemicals/CAS: adiponectin, 283182-39-8; alanine aminotransferase, 9000-86-6, 9014-30-6; aspartate aminotransferase, 9000-97-9; C reactive protein, 9007-41-4; cholesterol, 57-88-5; creatinine, 19230-81-0, 60-27-5; cyanocobalamin, 53570-76-6, 68-19-9, 8064-09-3; ferritin, 9007-73-2; folic acid, 59-30-3, 6484-89-5; gamma glutamyltransferase, 85876-02-4; glucose, 50-99-7, 84778-64-3; hemoglobin, 9008-02-0; hemoglobin A1c, 62572-11-6; insulin, 9004-10-8; liothyronine, 6138-47-2, 6893-02-3; proinsulin, 11062-00-3, 9035-68-1; thyrotropin, 9002-71-5; urea, 57-13-6; uric acid, 69-93-2; zinc, 7440-66-6, 14378-32-6; Biomarkers Funding details: 2014-7081-004 Funding details: Laurea University of Applied Sciences Funding text 1: This work was supported by the County of Salzburg (grant number 2014-7081-004) and an Institutional Grant of the Salzburg University of Applied Sciences. Funding text 2: Acknowledgments: We greatly acknowledge the coordination of the MoYo-project at the partner school by Edith Oberkofler and the competent assistance during sampling by Justine Gmachl-Baugartner, Renate Wiltsche and Liselotte Helminger. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission. Research funding: This work was supported by the County of Salzburg (grant number 2014-7081-004) and an Institutional Grant of the Salzburg University of Applied Sciences. Employment or leadership: None declared. Honorarium: None declared. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication. References: Jung, B., Adeli, K., Clinical laboratory reference intervals in pediatrics: The CALIPER initiative (2009) Clin Biochem, 42, pp. 1589-1595; Tahmasebi, H., Higgins, V., Fung, A.W., Truong, D., White-Al Habeeb, N.M., Adeli, K., Pediatric reference intervals for biochemical markers: Gaps and challenges, recent national initiatives and future perspectives (2017) EJIFCC, 28, pp. 43-63; Soldin, S.J., Wong, E.C., Brugnara, C., Soldin, O.P., (2011) Pediatric Reference Intervals, p. 304. , 7th ed. Washington, DC AACC Press; Ahrens, W., Bammann, K., Siani, A., Buchecker, K., De Henauw, S., Iacoviello, L., The IDEFICS cohort: Design, characteristics and participation in the baseline survey (2011) Int J Obes (Lond), 35, pp. S3-15; Bogner, B., Schwenoha, K., Gmeinhart, K., Tiefengrabner, M., Stutz, T., Ardelt-Gattinger, E., The MoYo-Project-study design and feasibility testing (2015) Appetite, 89, p. 321; (2010) WHO Guidelines on Drawing Blood: Best Practices in Phlebotomy, p. 125. , World Health Organization Geneva, Switzerland: WHO Press; (2008) Defining Establishing Verifying Reference Intervals in the Clinical Laboratory; Approved Guideline-third Edition in CLSI Document C28-A3c, , CLSI Wayne PA: Institute CaLS; Zierk, J., Arzideh, F., Haeckel, R., Rascher, W., Rauh, M., Metzler, M., Indirect determination of pediatric blood count reference intervals (2013) Clin Chem Lab Med, 51, pp. 863-872; Taylor, M.R., Holland, C.V., Spencer, R., Jackson, J.F., O'Connor, G.I., O'Donnell, J.R., Haematological reference ranges for schoolchildren (1997) Clin Lab Haematol, 19, pp. 1-15; Colantonio, D.A., Kyriakopoulou, L., Chan, M.K., Daly, C.H., Brinc, D., Venner, A.A., Closing the gaps in pediatric laboratory reference intervals: A CALIPER database of 40 biochemical markers in a healthy and multiethnic population of children (2012) Clin Chem, 58, pp. 854-868; Bailey, D., Colantonio, D., Kyriakopoulou, L., Cohen, A.H., Chan, M.K., Armbruster, D., Marked biological variance in endocrine and biochemical markers in childhood: Establishment of pediatric reference intervals using healthy community children from the CALIPER cohort (2013) Clin Chem, 59, pp. 1393-1405; Konforte, D., Shea, J.L., Kyriakopoulou, L., Colantonio, D., Cohen, A.H., Shaw, J., Complex biological pattern of fertility hormones in children and adolescents: A study of healthy children from the CALIPER cohort and establishment of pediatric reference intervals (2013) Clin Chem, 59, pp. 1215-1227; Rosario, A.S., Kurth, B.M., Stolzenberg, H., Ellert, U., Neuhauser, H., Body mass index percentiles for children and adolescents in Germany based on a nationally representative sample (KiGGS 2003-2006) (2010) Eur J Clin Nutr, 64, pp. 341-349; Bacopoulou, F., Efthymiou, V., Landis, G., Rentoumis, A., Chrousos, G.P., Waist circumference, waist-to-hip ratio and waist-to-height ratio reference percentiles for abdominal obesity among Greek adolescents (2015) BMC Pediatr, 15, p. 50; De Onis, M., Onyango, A.W., Borghi, E., Siyam, A., Nishida, C., Siekmann, J., Development of a WHO growth reference for schoolaged children and adolescents (2007) Bull World Health Organ, 85, pp. 660-667; Lee, C.M., Huxley, R.R., Wildman, R.P., Woodward, M., Indices of abdominal obesity are better discriminators of cardiovascular risk factors than BMI: A meta-analysis (2008) J Clin Epidemiol, 61, pp. 646-653; Zierk, J., Arzideh, F., Rechenauer, T., Haeckel, R., Rascher, W., Metzler, M., Age-And sex-specific dynamics in 22 hematologic and biochemical analytes from birth to adolescence (2015) Clin Chem, 61, pp. 964-973; Dortschy, R., Schaffrath-Rosario, A., Scheidt-Nave, C., Thierfelder, W., Thamm, M., Gutsche, J., Bevolkerungsbezogene Verteilungswerte ausgewahlter Laborparameter aus der Studie zur Gesundheit von Kindern und Jugendlichen in Deutschland (KiGGS) (2009) Beitrage Zur Gesundheitsberichterstattung des Bundes Koch-Institut R Editor, 132. , Robert Koch-Institut: Germany; Ceriotti, F., Boyd, J.C., Klein, G., Henny, J., Queralto, J., Kairisto, V., Reference intervals for serum creatinine concentrations: Assessment of available data for global application (2008) Clin Chem, 54, pp. 559-566; Bailey, D., Bevilacqua, V., Colantonio, D.A., Pasic, M.D., Perumal, N., Chan, M.K., Pediatric within-day biological variation and quality specifications for 38 biochemical markers in the CALIPER cohort (2014) Clin Chem, 60, pp. 518-529; Bevilacqua, V., Chan, M.K., Chen, Y., Armbruster, D., Schodin, B., Adeli, K., Pediatric population reference value distributions for cancer biomarkers and covariate-stratified reference intervals in the CALIPER cohort (2014) Clin Chem, 60, pp. 1532-1542; Teodoro-Morrison, T., Kyriakopoulou, L., Chen, Y.K., Raizman, J.E., Bevilacqua, V., Chan, M.K., Dynamic biological changes in metabolic disease biomarkers in childhood and adolescence: A CALIPER study of healthy community children (2015) Clin Biochem, 48, pp. 828-836; (2013) Method Procedure Comparison and Bias Estimation Using Patient Samples; Approved Guideline-Third Edition, , CLSI CLSI document EP09-A3. Wayne, PA: Institute CaLS; Langer, C., Tambyrayah, B., Thedieck, S., Nowak-Gottl, U., Testing for lipoprotein(a) concentration and apolipoprotein(a) phenotypes: Method standardization and pediatric reference values (2011) Semin Thromb Hemost, 37, pp. 810-813; Lausten-Thomsen, U., Christiansen, M., Fonvig, C.E., Trier, C., Pedersen, O., Hansen, T., Reference values for serum total adiponectin in healthy non-obese children and adolescents (2015) Clin Chim Acta, 450, pp. 11-14",

year = "2018",

month = oct,

day = "30",

doi = "10.1515/cclm-2018-0715",

language = "English",

volume = "57",

pages = "891--900",

journal = "Clinical Chemistry and Laboratory Medicine",

issn = "1434-6621",

publisher = "Walter de Gruyter GmbH",

number = "6",

}

TY - JOUR

T1 - Evaluation of reference intervals of haematological and biochemical markers in an Austrian adolescent study cohort

AU - Bogner, B.

AU - Schwenoha, K.

AU - Vogl, M.

AU - Weghuber, D.

AU - Roth, C.

AU - Kipman, U.

AU - Cadamuro, J.

AU - Oostingh, G.J.

N1 - Cited By :4 Export Date: 14 December 2023 CODEN: CCLMF Correspondence Address: Bogner, B.; Salzburg University of Applied Sciences, Urstein Süd 1, Austria; email: [email protected] Chemicals/CAS: adiponectin, 283182-39-8; alanine aminotransferase, 9000-86-6, 9014-30-6; aspartate aminotransferase, 9000-97-9; C reactive protein, 9007-41-4; cholesterol, 57-88-5; creatinine, 19230-81-0, 60-27-5; cyanocobalamin, 53570-76-6, 68-19-9, 8064-09-3; ferritin, 9007-73-2; folic acid, 59-30-3, 6484-89-5; gamma glutamyltransferase, 85876-02-4; glucose, 50-99-7, 84778-64-3; hemoglobin, 9008-02-0; hemoglobin A1c, 62572-11-6; insulin, 9004-10-8; liothyronine, 6138-47-2, 6893-02-3; proinsulin, 11062-00-3, 9035-68-1; thyrotropin, 9002-71-5; urea, 57-13-6; uric acid, 69-93-2; zinc, 7440-66-6, 14378-32-6; Biomarkers Funding details: 2014-7081-004 Funding details: Laurea University of Applied Sciences Funding text 1: This work was supported by the County of Salzburg (grant number 2014-7081-004) and an Institutional Grant of the Salzburg University of Applied Sciences. Funding text 2: Acknowledgments: We greatly acknowledge the coordination of the MoYo-project at the partner school by Edith Oberkofler and the competent assistance during sampling by Justine Gmachl-Baugartner, Renate Wiltsche and Liselotte Helminger. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission. Research funding: This work was supported by the County of Salzburg (grant number 2014-7081-004) and an Institutional Grant of the Salzburg University of Applied Sciences. Employment or leadership: None declared. Honorarium: None declared. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication. References: Jung, B., Adeli, K., Clinical laboratory reference intervals in pediatrics: The CALIPER initiative (2009) Clin Biochem, 42, pp. 1589-1595; Tahmasebi, H., Higgins, V., Fung, A.W., Truong, D., White-Al Habeeb, N.M., Adeli, K., Pediatric reference intervals for biochemical markers: Gaps and challenges, recent national initiatives and future perspectives (2017) EJIFCC, 28, pp. 43-63; Soldin, S.J., Wong, E.C., Brugnara, C., Soldin, O.P., (2011) Pediatric Reference Intervals, p. 304. , 7th ed. Washington, DC AACC Press; Ahrens, W., Bammann, K., Siani, A., Buchecker, K., De Henauw, S., Iacoviello, L., The IDEFICS cohort: Design, characteristics and participation in the baseline survey (2011) Int J Obes (Lond), 35, pp. S3-15; Bogner, B., Schwenoha, K., Gmeinhart, K., Tiefengrabner, M., Stutz, T., Ardelt-Gattinger, E., The MoYo-Project-study design and feasibility testing (2015) Appetite, 89, p. 321; (2010) WHO Guidelines on Drawing Blood: Best Practices in Phlebotomy, p. 125. , World Health Organization Geneva, Switzerland: WHO Press; (2008) Defining Establishing Verifying Reference Intervals in the Clinical Laboratory; Approved Guideline-third Edition in CLSI Document C28-A3c, , CLSI Wayne PA: Institute CaLS; Zierk, J., Arzideh, F., Haeckel, R., Rascher, W., Rauh, M., Metzler, M., Indirect determination of pediatric blood count reference intervals (2013) Clin Chem Lab Med, 51, pp. 863-872; Taylor, M.R., Holland, C.V., Spencer, R., Jackson, J.F., O'Connor, G.I., O'Donnell, J.R., Haematological reference ranges for schoolchildren (1997) Clin Lab Haematol, 19, pp. 1-15; Colantonio, D.A., Kyriakopoulou, L., Chan, M.K., Daly, C.H., Brinc, D., Venner, A.A., Closing the gaps in pediatric laboratory reference intervals: A CALIPER database of 40 biochemical markers in a healthy and multiethnic population of children (2012) Clin Chem, 58, pp. 854-868; Bailey, D., Colantonio, D., Kyriakopoulou, L., Cohen, A.H., Chan, M.K., Armbruster, D., Marked biological variance in endocrine and biochemical markers in childhood: Establishment of pediatric reference intervals using healthy community children from the CALIPER cohort (2013) Clin Chem, 59, pp. 1393-1405; Konforte, D., Shea, J.L., Kyriakopoulou, L., Colantonio, D., Cohen, A.H., Shaw, J., Complex biological pattern of fertility hormones in children and adolescents: A study of healthy children from the CALIPER cohort and establishment of pediatric reference intervals (2013) Clin Chem, 59, pp. 1215-1227; Rosario, A.S., Kurth, B.M., Stolzenberg, H., Ellert, U., Neuhauser, H., Body mass index percentiles for children and adolescents in Germany based on a nationally representative sample (KiGGS 2003-2006) (2010) Eur J Clin Nutr, 64, pp. 341-349; Bacopoulou, F., Efthymiou, V., Landis, G., Rentoumis, A., Chrousos, G.P., Waist circumference, waist-to-hip ratio and waist-to-height ratio reference percentiles for abdominal obesity among Greek adolescents (2015) BMC Pediatr, 15, p. 50; De Onis, M., Onyango, A.W., Borghi, E., Siyam, A., Nishida, C., Siekmann, J., Development of a WHO growth reference for schoolaged children and adolescents (2007) Bull World Health Organ, 85, pp. 660-667; Lee, C.M., Huxley, R.R., Wildman, R.P., Woodward, M., Indices of abdominal obesity are better discriminators of cardiovascular risk factors than BMI: A meta-analysis (2008) J Clin Epidemiol, 61, pp. 646-653; Zierk, J., Arzideh, F., Rechenauer, T., Haeckel, R., Rascher, W., Metzler, M., Age-And sex-specific dynamics in 22 hematologic and biochemical analytes from birth to adolescence (2015) Clin Chem, 61, pp. 964-973; Dortschy, R., Schaffrath-Rosario, A., Scheidt-Nave, C., Thierfelder, W., Thamm, M., Gutsche, J., Bevolkerungsbezogene Verteilungswerte ausgewahlter Laborparameter aus der Studie zur Gesundheit von Kindern und Jugendlichen in Deutschland (KiGGS) (2009) Beitrage Zur Gesundheitsberichterstattung des Bundes Koch-Institut R Editor, 132. , Robert Koch-Institut: Germany; Ceriotti, F., Boyd, J.C., Klein, G., Henny, J., Queralto, J., Kairisto, V., Reference intervals for serum creatinine concentrations: Assessment of available data for global application (2008) Clin Chem, 54, pp. 559-566; Bailey, D., Bevilacqua, V., Colantonio, D.A., Pasic, M.D., Perumal, N., Chan, M.K., Pediatric within-day biological variation and quality specifications for 38 biochemical markers in the CALIPER cohort (2014) Clin Chem, 60, pp. 518-529; Bevilacqua, V., Chan, M.K., Chen, Y., Armbruster, D., Schodin, B., Adeli, K., Pediatric population reference value distributions for cancer biomarkers and covariate-stratified reference intervals in the CALIPER cohort (2014) Clin Chem, 60, pp. 1532-1542; Teodoro-Morrison, T., Kyriakopoulou, L., Chen, Y.K., Raizman, J.E., Bevilacqua, V., Chan, M.K., Dynamic biological changes in metabolic disease biomarkers in childhood and adolescence: A CALIPER study of healthy community children (2015) Clin Biochem, 48, pp. 828-836; (2013) Method Procedure Comparison and Bias Estimation Using Patient Samples; Approved Guideline-Third Edition, , CLSI CLSI document EP09-A3. Wayne, PA: Institute CaLS; Langer, C., Tambyrayah, B., Thedieck, S., Nowak-Gottl, U., Testing for lipoprotein(a) concentration and apolipoprotein(a) phenotypes: Method standardization and pediatric reference values (2011) Semin Thromb Hemost, 37, pp. 810-813; Lausten-Thomsen, U., Christiansen, M., Fonvig, C.E., Trier, C., Pedersen, O., Hansen, T., Reference values for serum total adiponectin in healthy non-obese children and adolescents (2015) Clin Chim Acta, 450, pp. 11-14

PY - 2018/10/30

Y1 - 2018/10/30

N2 - Background Reference intervals are a prerequisite for the interpretation of laboratory data related to diagnostic issues and treatment strategies. In adolescents, biomarker concentrations change with age, necessitating a continuous age-related definition of the reference intervals. The purpose of this pilot study was to evaluate the reference intervals for a healthy population of adolescents in Salzburg and compare these, when possible, with age- and gender-matched published data. Methods Anthropometrical parameters and blood samples were collected from adolescents (male and female; 14–17 years) in a school setting. Haematological samples were measured using Sysmex XS-1000i, lipid and carbohydrate metabolism markers as well as enzymes and hormones were determined by Cobas c311, Vitros ECiQ® or ELISA. The reference intervals were calculated according to the CLSI guidelines C28-A3c. Results Samples of 102 participants were included. Compared to age- and gender-matched reference intervals, the BMI levels were in the lower normal rage. Most haematological parameters and biomedical makers reveal similar ranges to values published in other studies. Conclusions This data analysis allowed for a partial comparison of reference values with published data and enabled a new determination of paediatric reference intervals for an Austrian cohort. © 2019 Barbara Bogner et al., published by De Gruyter, Berlin/Boston.

AB - Background Reference intervals are a prerequisite for the interpretation of laboratory data related to diagnostic issues and treatment strategies. In adolescents, biomarker concentrations change with age, necessitating a continuous age-related definition of the reference intervals. The purpose of this pilot study was to evaluate the reference intervals for a healthy population of adolescents in Salzburg and compare these, when possible, with age- and gender-matched published data. Methods Anthropometrical parameters and blood samples were collected from adolescents (male and female; 14–17 years) in a school setting. Haematological samples were measured using Sysmex XS-1000i, lipid and carbohydrate metabolism markers as well as enzymes and hormones were determined by Cobas c311, Vitros ECiQ® or ELISA. The reference intervals were calculated according to the CLSI guidelines C28-A3c. Results Samples of 102 participants were included. Compared to age- and gender-matched reference intervals, the BMI levels were in the lower normal rage. Most haematological parameters and biomedical makers reveal similar ranges to values published in other studies. Conclusions This data analysis allowed for a partial comparison of reference values with published data and enabled a new determination of paediatric reference intervals for an Austrian cohort. © 2019 Barbara Bogner et al., published by De Gruyter, Berlin/Boston.

KW - adolescents

KW - laboratory parameters

KW - paediatrics

KW - post-analytics

KW - reference intervals

KW - adiponectin

KW - alanine aminotransferase

KW - albumin

KW - apolipoprotein A1

KW - apolipoprotein B

KW - aspartate aminotransferase

KW - biochemical marker

KW - C reactive protein

KW - cholesterol

KW - creatinine

KW - cyanocobalamin

KW - cystatin C

KW - ferritin

KW - folic acid

KW - gamma glutamyltransferase

KW - glucose

KW - hemoglobin

KW - hemoglobin A1c

KW - high density lipoprotein cholesterol

KW - insulin

KW - liothyronine

KW - lipoprotein A

KW - low density lipoprotein cholesterol

KW - proinsulin

KW - thyrotropin

KW - triacylglycerol

KW - urea

KW - uric acid

KW - vitamin D

KW - zinc

KW - biological marker

KW - adolescent

KW - alanine aminotransferase blood level

KW - albumin blood level

KW - Article

KW - aspartate aminotransferase blood level

KW - Austrian

KW - basophil

KW - body mass

KW - cholesterol blood level

KW - cohort analysis

KW - controlled study

KW - creatinine blood level

KW - eosinophil count

KW - erythrocyte count

KW - female

KW - ferritin blood level

KW - free liothyronine index

KW - free thyroxine index

KW - gamma glutamyl transferase blood level

KW - glucose blood level

KW - hematocrit

KW - hematological parameters

KW - hemoglobin blood level

KW - human

KW - insulin blood level

KW - leukocyte count

KW - lipoprotein blood level

KW - lymphocyte count

KW - major clinical study

KW - male

KW - mean corpuscular hemoglobin

KW - mean corpuscular volume

KW - monocyte count

KW - neutrophil count

KW - pilot study

KW - platelet count

KW - priority journal

KW - protein blood level

KW - reference value

KW - thyrotropin blood level

KW - triacylglycerol blood level

KW - urea blood level

KW - uric acid blood level

KW - vitamin blood level

KW - zinc blood level

KW - Austria

KW - blood

KW - enzyme assay

KW - enzyme linked immunosorbent assay

KW - normal human

KW - waist hip ratio

KW - Adolescent

KW - Biomarkers

KW - Body Mass Index

KW - Cohort Studies

KW - Enzyme Assays

KW - Enzyme-Linked Immunosorbent Assay

KW - Female

KW - Healthy Volunteers

KW - Humans

KW - Male

KW - Reference Values

KW - Waist-Hip Ratio

UR - https://www.mendeley.com/catalogue/6da7ac58-85a3-3489-a6ea-9e96a83b09b8/

U2 - 10.1515/cclm-2018-0715

DO - 10.1515/cclm-2018-0715

M3 - Article

SN - 1434-6621

VL - 57

SP - 891

EP - 900

JO - Clinical Chemistry and Laboratory Medicine

JF - Clinical Chemistry and Laboratory Medicine

IS - 6

ER -

Evaluation of reference intervals of haematological and biochemical markers in an Austrian adolescent study cohort

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