TY - JOUR
T1 - Determination of nitration degrees for the birch pollen allergen Bet v 1
AU - Selzle, K.
AU - Ackaert, C.
AU - Kampf, C.J.
AU - Kunert, A.T.
AU - Duschl, A.
AU - Oostingh, G.J.
AU - P̈öschl, U.
N1 - Cited By :20
Export Date: 14 December 2023
CODEN: ABCNB
Correspondence Address: Oostingh, G.J.; Biomedical Sciences, , 5412 Puch/ Salzburg, Austria; email: [email protected]
Chemicals/CAS: 3 nitrotyrosine, 3604-79-3; peroxynitrous acid, 14691-52-2; tyrosine, 16870-43-2, 55520-40-6, 60-18-4
Funding details: Austrian Science Fund, FWF, P22236-B13
Funding details: Johannes Gutenberg-Universität Mainz, JGU
Funding details: Max-Planck-Gesellschaft, MPG
Funding text 1: Acknowledgments This work was funded by the Max Planck Society (MPG) and the Austrian Science Fund (FWF): P22236-B13. KS is supported by the Max Planck Graduate Center–Johannes Gutenberg University Mainz (MPGC-JOGU). The authors gratefully acknowledge T. Pooya and G. Gadermaier for the technical support, M. Reinmuth for the fruitful discussions, and M.O. Andreae for the support. We appreciate the support by S. Kofler, F. Ferreira, and H. Brandstetter for providing the Bet v 1.0101.
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PY - 2013
Y1 - 2013
N2 - Nitration of tyrosine residues in the major birch pollen allergen Bet v 1 may alter the allergenic potential of the protein. The kinetics and mechanism of the nitration reaction, however, have not yet been well characterized. To facilitate further investigations, an efficientmethod to quantify the nitration degree (ND) of small samples of Bet v 1 is required. Here, we present a suitable method of highperformance liquid chromatography coupled to a diode array detector (HPLC-DAD) that can be photometrically calibrated using the amino acids tyrosine (Tyr) and nitrotyrosine (NTyr) without the need for nitrated protein standards. The new method is efficient and in agreement with alternative methods based on hydrolysis and amino acid analysis of tetranitromethane (TNM)-nitrated Bet v 1 standards as well as samples from nitration experiments with peroxynitrite. The results confirm the applicability of the new method for the investigation of the reaction kinetics and mechanism of protein nitration. © Springer-Verlag Berlin Heidelberg 2013.
AB - Nitration of tyrosine residues in the major birch pollen allergen Bet v 1 may alter the allergenic potential of the protein. The kinetics and mechanism of the nitration reaction, however, have not yet been well characterized. To facilitate further investigations, an efficientmethod to quantify the nitration degree (ND) of small samples of Bet v 1 is required. Here, we present a suitable method of highperformance liquid chromatography coupled to a diode array detector (HPLC-DAD) that can be photometrically calibrated using the amino acids tyrosine (Tyr) and nitrotyrosine (NTyr) without the need for nitrated protein standards. The new method is efficient and in agreement with alternative methods based on hydrolysis and amino acid analysis of tetranitromethane (TNM)-nitrated Bet v 1 standards as well as samples from nitration experiments with peroxynitrite. The results confirm the applicability of the new method for the investigation of the reaction kinetics and mechanism of protein nitration. © Springer-Verlag Berlin Heidelberg 2013.
KW - Bet v 1.0101
KW - HPLC-DAD
KW - Nitration degree
KW - Protein tyrosine nitration
KW - Allergens
KW - Amino acids
KW - Liquid chromatography
KW - Proteins
KW - Reaction kinetics
KW - Amino acid analysis
KW - Diode array detectors
KW - Kinetics and mechanism
KW - Nitration reactions
KW - Protein tyrosine nitrations
KW - Tyrosine residues
KW - Nitration
KW - 3 nitrotyrosine
KW - 3-nitrotyrosine
KW - Bet v 1 allergen, Betula
KW - drug derivative
KW - peroxynitrous acid
KW - plant antigen
KW - recombinant protein
KW - tyrosine
KW - vegetable protein
KW - article
KW - birch
KW - calibration
KW - chemistry
KW - high performance liquid chromatography
KW - immunology
KW - pollen
KW - protein processing
KW - Antigens, Plant
KW - Betula
KW - Calibration
KW - Chromatography, High Pressure Liquid
KW - Peroxynitrous Acid
KW - Plant Proteins
KW - Pollen
KW - Protein Processing, Post-Translational
KW - Recombinant Proteins
KW - Tyrosine
U2 - 10.1007/s00216-013-7324-0
DO - 10.1007/s00216-013-7324-0
M3 - Article
SN - 1618-2642
VL - 405
SP - 8945
EP - 8949
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
IS - 27
ER -